How to use the online web-server:
Step 1. Either type or copy/paste the query protein sequences into the input box. The input sequence should be in the FASTA format. Example sequences in FASTA format can be seen by clicking on the Example button right above the input box. For more information about FASTA format, visit

Step 2. Click on the Submit button to see the predicted result. For example, if you use the query protein sequences in the Example window as the input, after clicking the Submit button, you will see on your screen the predicted glycated site positions and the corresponding sequences segments with the form as formulated by Eq.1. It takes about a few seconds for the above computation before the predicted results appear on the computer screen; the more number of query proteins and longer of each sequence, the more time it is usually needed.

Step 3. You may also choose the prediction by entering your desired input file via the "Browse" button. The input file should also be in FASTA format but can contain as many protein sequences as you want.

Step 4. Click on the Citation button to find the relevant papers that document the detailed development and algorithm of Gly-PseAAC.

Step 5. Click on the Data button to download the benchmark datasets used to train and test the Gly-PseAAC predictor.

1.To obtain the predicted results , please input the query protein sequences.

2.The accepted characters are: A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, and the dummy code X. If the query sequence contains any illegal characters, the prediction will be stopped.

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